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Cosmo Bio USA polymorphprep gradient medium axs-1114683
Polymorphprep Gradient Medium Axs 1114683, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+gradient/polymorphprep+gradient+medium/pmc12066692-46-9-12
Average 90 stars, based on 1 article reviews
polymorphprep gradient medium axs-1114683 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: Extensive citrullination of human serum albumin is physiological and not inherently immunogenic in rheumatoid arthritis.
Article Snippet: Horse radish peroxidase (HRP)-conjugated goat anti-human IgG was from ImmunoReagents, 3,3’,5,5’- tetramethylbenzidine (TMB) from BioLegend (#50-169-261), in-gel tryptic digestion kit Jo na l P re- pro of 16 from ThermoFisher Scientific (#89871X), EasyPep in-solution tryptic digestion kit from ThermoFisher Scientific (#A40006), 96% purified human serum albumin from ThermoFisher Scientific (#J66780.03), human AB Rh+ serum, heat-inactivated at 56°C, from Atlanta Biologicals (now R&D Systems) (#S40110H), recombinant PAD2 and PAD4 (Cayman), Pierce protein G agarose beads from ThermoFisher Scientific (#20397), AntiCitrulline (Modified) Detection Kit from Sigma-Aldrich (#17-346B-1), Thyroxine Competitive ELISA kit from Invitrogen/ThermoFisher Scientific (#EIATAC), and Immunoscan CCPlus from Svar Life Science AB (RA-96PLUS). .. Neutrophils were isolated from venous blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer's instructions. .. They were subsequently washed and suspended in DMEM high glucose (#11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils
Article Snippet: .. Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol . ..

Article Title: Pregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation.
Article Snippet: .. Polymorphonuclear leukocytes (PMNs) were isolated using the Polymorphprep density gradient (Cosmo Bio USA, Inc., Carlsbad, CA, USA; cat# AXS1114683), per the manufacturer’s instructions. ..

Article Title: Interferon-dependent signaling is critical for viral clearance in airway neutrophils
Article Snippet: Data were analyzed using the IDEAS software v6.1 (Luminex Corporation). .. In vitro transmigration Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol (16). .. Neutrophil activation, purity (99% purity was obtained for every isolation), and viability was assessed using flow cytometry. and allowed to migrate into the chemoattractant LTB4 (100nM, Sigma) (A1 neutrophils, (16)) or into ALI/ARDS mBAL supernatant diluted 1:1 in plain RPMI (A2 neutrophils).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes.
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).

Gradient Centrifugation:

Article Title: Extensive citrullination of human serum albumin is physiological and not inherently immunogenic in rheumatoid arthritis.
Article Snippet: Horse radish peroxidase (HRP)-conjugated goat anti-human IgG was from ImmunoReagents, 3,3’,5,5’- tetramethylbenzidine (TMB) from BioLegend (#50-169-261), in-gel tryptic digestion kit Jo na l P re- pro of 16 from ThermoFisher Scientific (#89871X), EasyPep in-solution tryptic digestion kit from ThermoFisher Scientific (#A40006), 96% purified human serum albumin from ThermoFisher Scientific (#J66780.03), human AB Rh+ serum, heat-inactivated at 56°C, from Atlanta Biologicals (now R&D Systems) (#S40110H), recombinant PAD2 and PAD4 (Cayman), Pierce protein G agarose beads from ThermoFisher Scientific (#20397), AntiCitrulline (Modified) Detection Kit from Sigma-Aldrich (#17-346B-1), Thyroxine Competitive ELISA kit from Invitrogen/ThermoFisher Scientific (#EIATAC), and Immunoscan CCPlus from Svar Life Science AB (RA-96PLUS). .. Neutrophils were isolated from venous blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer's instructions. .. They were subsequently washed and suspended in DMEM high glucose (#11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

Article Title: Subcellular location of L1 retrotransposon-encoded ORF1p, reverse transcription products, and DNA sensors in lupus granulocytes.
Article Snippet: .. Neutrophils were isolated from freshly drawn blood by gradient centrifugation on PolymorphPrep (CosmoBio, USA) according to the manufacturer’s instructions. .. The cells were washed and suspended in DMEM high glucose (# 11-965-118, Gibco, ThermoFisher Scientific).

In Vitro:

Article Title: Interferon-dependent signaling is critical for viral clearance in airway neutrophils
Article Snippet: Data were analyzed using the IDEAS software v6.1 (Luminex Corporation). .. In vitro transmigration Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol (16). .. Neutrophil activation, purity (99% purity was obtained for every isolation), and viability was assessed using flow cytometry. and allowed to migrate into the chemoattractant LTB4 (100nM, Sigma) (A1 neutrophils, (16)) or into ALI/ARDS mBAL supernatant diluted 1:1 in plain RPMI (A2 neutrophils).

Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).

Transmigration Assay:

Article Title: Interferon-dependent signaling is critical for viral clearance in airway neutrophils
Article Snippet: Data were analyzed using the IDEAS software v6.1 (Luminex Corporation). .. In vitro transmigration Neutrophils were isolated from whole blood using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol (16). .. Neutrophil activation, purity (99% purity was obtained for every isolation), and viability was assessed using flow cytometry. and allowed to migrate into the chemoattractant LTB4 (100nM, Sigma) (A1 neutrophils, (16)) or into ALI/ARDS mBAL supernatant diluted 1:1 in plain RPMI (A2 neutrophils).

Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).

Flow Cytometry:

Article Title: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.
Article Snippet: .. In vitro transmigration Blood neutrophils were isolated using the density gradient Polymorphprep (Cosmo Bio USA) following manufacturer protocol.48 Neutrophil purity and viability was assessed using flow cytometry. .. Purified neutrophils were loaded in the in vitro transmigration model18 as follows: 1) transmigration toward the neutrophil chemoattractant LTB4 (100nM, Sigma) (transmigrated neutrophils not undergoing GRIM adaptation); 2) transmigration using the CF airway supernatant (CFASN) generated from sputum as apical fluid, (transmigrated neutrophils undergoing GRIM adaptation); 3) transmigration using the COPD airway supernatant (COPDASN) generated from sputum as apical fluid; 4) CXCL-8 (Biolegend, 10 ng/mL); 5) LPS (Sigma, 10 ng/mL); and C5a (R&D systems, 500 ng/mL).



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